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PH0613 | Tris-硼酸电泳缓冲液(5×TBE Buffer) 
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货号: PH0613 品牌: Phygene
规格: 500ML 单位:
容量: 500ML

货号 PH0613

名称 Tris-硼酸电泳缓冲液(5×TBE)

规格 500mL

存储 室温储存,有效期至少12 个月。


产品简介


TBE 缓冲液是生物学中常使用的核酸电泳缓冲盐溶液,主要用于 DNA 的琼脂糖凝胶电泳。TBE 的主要成分是 Tris-硼酸盐与 EDTA,缓冲能力强,适合较长时间电泳,分辨率较高,电泳小于 1kb 的片段时分离效果好。


TBE 缓冲液中的硼酸成分会影响 DNA 回收效率以及后续的酶反应,若要进行 DNA 片段的琼脂糖凝胶电泳回收实验,建议使用 TAE 缓冲液。


本产品为 5×浓缩液,工作浓度为 0.5×,可直接用蒸馏水稀释 10 倍后使用。若产品出现沉淀析出,请置于37℃水浴使其溶解,不影响使用。


About TBE Buffer

Tris buffers are used under slightly basic pH conditions, as for DNA electrophoresis, because this keeps the DNA soluble in the solution and deprotonated so it will be attracted to the positive electrode and will migrate through a gel. EDTA is an ingredient in the solution because this common chelating agent protects nucleic acids from degradation by enzymes. The EDTA chelates divalent cations that are cofactors for nucleases that may contaminate the sample. However, since the magnesium cation is a cofactor for DNA polymerase and restriction enzymes, the concentration of EDTA is kept purposely low (aroun 1 mM concentration).

Although TBE and TAE are common electrophoresis buffers, there are other options for low-molarity conductive solutions, including lithium borate buffer and sodium borate buffer. The problem with TBE and TAE is that Tris-based buffers limit the electric field that can be used in electrophoresis because too much charge causes a runaway temperature.


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